Journal: bioRxiv

Article Title: A DNA foundation model predicts osteoporosis risk genes without proximity bias

doi: 10.64898/2026.03.09.707383

Figure Lengend Snippet: a. All variant–gene pairs within a receptive field of 256kbp are scored by Rosalind. Genes above the elbow threshold are kept as candidate causal genes. b. The distribution of protein-coding genes around the 1103 lead variants from the eBMD GWAS (Morris et al. ) when using a window of 256kbp. c. Overlap of Rosalind-predicted genes with nearest genes is shown, with genes known to regulate eBMD highlighted. d. Mineralization assay experimental workflow: arrayed CRISPR/Cas9 knock-out screen of hFOB1.19 human osteoblasts. e. Plot shows SNP RSID-gene pairs, where at least one hit was observed in the osteoblast mineralisation assay. Hit calling was performed using Region of Practical Equivalence (ROPE) analysis and results show hits at ROPE 0.5 or greater (‘moderate’ effect size) f. Experimental validation summary, with assay “hits” represented in blue and “no effect” represented in red.

Article Snippet: hFOB1.19 (ATCC) were cultured in flasks in DMEM/F-12, 10% FBS (Sigma F7524 lot.0001663052), antibiotic/antimycotic (Gibco 15240-062) and G418 sulfate (ab144261) at 34oC until 80-90% confluent.

Techniques: Variant Assay, Mineralization Assay, CRISPR, Knock-Out, Biomarker Discovery

Journal: International Journal of Nanomedicine

Article Title: Electrospun nanofiber blend with improved mechanical and biological performance

doi: 10.2147/IJN.S175619

Figure Lengend Snippet: ( A ) ALP activity showed an increase of calcification of the extracellular matrix after inclusion of GelMA. ( B ) Calcium deposition demonstrated a further influence of GelMA to enhance the functions of osteoblasts. ( C ) MTS assay showing that osteoblastic cells were further influenced by hydrophilic properties after inclusion of PEG and GelMA. Data plotted in mean and SD (N=5). Values of P <0.01 were considered significant. Data were normalized by the cells, and the y-axis was multiplied by 10 4 . For the ALP and calcium deposition, the data were compared to control (cells) and between each time. For cellular proliferation assays, the data were compared to pure PCL. N=5. ** P <0.01, *** P <0.001, and **** P <0.0001 mean statistical differences. SEM of hFOBs cultivated on scaffolds after 7 days. ( D ) (i) PCL and (ii) magnified view. ( E ) (i) PCL-PEG and (ii) magnified view. ( F ) (i) PCL-PEG-GelMA without UV crosslinking and (ii) magnified view. ( G ) (i) PCL-PEG-GelMA after UV crosslinking and (ii) magnified view. The cells are spreading on all produced scaffolds presenting filopodium and cytoplasmic extension. Abbreviations: ALP, alkaline phosphatase; GelMA, gelatin methacryloyl; hFOB, human osteoblasts; MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); NS, no significance; PCL, polycaprolactone; PEG, poly(ethylene glycol); SEM, scanning electron microscopy.

Article Snippet: Totally 5,000 cells/cm 2 of human osteoblast cells line (hFOB 1.19, bone-forming cells; Lonza, CRL-11372, second passage) were cultured using C27015 media (Osteoblast Basal Medium, Promocell GmbH), 1% penicillin/streptomycin (P/S; Hyclone), and an Osteoblast Growth Medium Supplement Mix under standard cell culture conditions (37°C, 5% CO 2 , and 95% air).

Techniques: Activity Assay, MTS Assay, Control, Produced, Electron Microscopy

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques:

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques: Binding Assay, Expressing, Western Blot