Journal: PLoS ONE

Article Title: Mechanical and In Vitro Biological Performance of Graphene Nanoplatelets Reinforced Calcium Silicate Composite

doi: 10.1371/journal.pone.0106802

Figure Lengend Snippet: Proliferation of hFOB osteoblasts on different GNP/CS composites in comparison with pure CS and blank assessed using MTT assay ( p <0.05, n = 5).

Article Snippet: The human osteoblast cell lines (hFOB 1.19 SV40 transfected osteoblasts, ATCC, Rockville, MD, USA) were seeded on the sterilized pure CS and GNP/CS composites samples at a density of 1×10 4 cells ml −1 in 96-well culture plates.

Techniques: Comparison, MTT Assay

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Cell viability (%) of MG63 and Saos-2 cells treated with pexidartinib (via PLX3397) and sotuletinib.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques:

Journal: Oncology Letters

Article Title: Computational discovery of novel colony-stimulating factor-1 receptor as a potential therapeutic biomarker in osteosarcoma and a novel inhibitor from herbal sources

doi: 10.3892/ol.2026.15618

Figure Lengend Snippet: Sensitization of the MG63 and Saos-2 cell lines to a CSF1R inhibitor. (A) Dose-dependent cytotoxic effects of linarin on MG63 and Saos-2 osteosarcoma cell viability. (B) Dose-dependent cytotoxic effects of saikosaponin C on MG63 and Saos-2 osteosarcoma cell viability. (C) Dose-dependent cytotoxic effects of sarsasapogenin on MG63 and Saos-2 osteosarcoma cell viability. (D) Molecular docking of the binding between sarsasapogenin and the CSF1R protein. (E) Visual molecular docking of the binding acid residues between sarsasapogenin and CSF1R protein. (F) The expression levels of CSF1R as detected by western blot analysis in MG63 and Saos-2 cells after treatment with sarsasapogenin. *P<0.05. CSF1R, colony-stimulating factor-1 receptor.

Article Snippet: MG63 osteosarcoma cells were purchased from Procell Life Science Technology Co., Ltd. (cat. no. #CL-0157; http://www.procell.com.cn/p/mg-63-cl-0157-72575 ).

Techniques: Binding Assay, Expressing, Western Blot

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control

Journal: Translational Cancer Research

Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

doi: 10.21037/tcr-2025-aw-2290

Figure Lengend Snippet: The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

Techniques: Expressing, Transfection, Negative Control, Over Expression